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human aortic smooth muscle cells smcs  (ATCC)


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    ATCC human aortic smooth muscle cells smcs
    Human Aortic Smooth Muscle Cells Smcs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+smcs/T%2FG+HA-VSMC/pmc13019204-95-4-12
    Average 96 stars, based on 1111 article reviews
    human aortic smooth muscle cells smcs - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Differential effects of traditional cigarette, electronic cigarette, and tobacco heating product on human vascular smooth muscle cell phenotypic switch: A comparative functional perspective.
    Article Snippet: .. Human aortic SMCs (HASMC; PCS-100-012) were purchased from ATCC (Manassas, FL, USA), and cultured in ATCC Vascular Cell Basal Medium (PCS-100-030, ATCC; 500 ml added with 500 μl ascorbic acid, 500 μl rh EGF, 500 μl rh insulin and rh FGF-b, 25 ml glutamine), 5 % fetal bovine serum (FBS, ATCC Vascular Smooth Muscle Growth Kit), and 5 ml Penicillin-Streptomycin 100X (Euroclone, Milan, Italy). ..

    Article Title: Adipose-derived stromal cell secreted factors induce the elastogenesis cascade within 3D aortic smooth muscle cell constructs
    Article Snippet: .. Human aortic SMCs (ATCC #PCS-100-012, Manassas, Virginia) were cultured at 37 °C and 5% CO 2 , with growth media (#311K-500, Cell Applications Inc., San Diego, CA) changes every 48–72 h. Cells used in experimental cultures were between passages 4 and 12. .. SMC-seeded fibrin gel constructs [ , ] were formed using 3.7 mg/mL bovine fibrinogen type I (Sigma-Aldrich #8630), 0.21 U/mL bovine thrombin (Sigma-Aldrich #T7513), and 5 × 10 5 SMCs/mL.

    Article Title: Smooth Muscle Cell Phenotypic Switch Induced by Traditional Cigarette Smoke Condensate: A Holistic Overview.
    Article Snippet: Subconfluent SMCs were incubated in DMEM (Euroclone, Milan, Italy) supplemented with 0.2% essential-fatty-acid-free albumin. .. Human aortic SMCs (PCS-100-012, ATCC, MA, USA) were cultured in ATCC Vascular Cell Basal Medium (PCS-100-030, ATCC; 500 mL supplemented with 500 μL ascorbic acid, 500 μL rh EGF, 500 μL rh insulin and rh FGF-b, 25 mL glutamine), 5% FBS (ATCC Vascular Smooth Muscle Growth kit), and 5 mL penicillin–streptomycin 100× (Euroclone, Milan, Italy). ..

    Article Title: Smooth Muscle Cell Phenotypic Switch Induced by Traditional Cigarette Smoke Condensate: A Holistic Overview
    Article Snippet: Subconfluent SMCs were incubated in DMEM (Euroclone, Milan, Italy) supplemented with 0.2% essential-fatty-acid-free albumin. .. Human aortic SMCs (PCS-100-012, ATCC, MA, USA) were cultured in ATCC Vascular Cell Basal Medium (PCS-100-030, ATCC; 500 mL supplemented with 500 μL ascorbic acid, 500 μL rh EGF, 500 μL rh insulin and rh FGF-b, 25 mL glutamine), 5% FBS (ATCC Vascular Smooth Muscle Growth kit), and 5 mL penicillin–streptomycin 100× (Euroclone, Milan, Italy). ..



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    A. Outline of the experiment. Human <t>aortic</t> <t>SMCs</t> were exposed to static conditions (24 h), mechanical stretch (10%, 6h) or TNF stimulation (10 ng/mL, 24h). Uniform Manifold Approximation and Projection (UMAP) visualization of the phenotypic diversity of SMCs after static culture, stretch and TNF stimulation. Each dot represents a single cell. Nine cell clusters were identified in human aortic SMCs (0.3 resolution) as follows: C0 (fibromyocyte SMCs), C1 (inflammatory SMCs), C2 (proliferative M-phase), C3 (intermediate SMCs), C4 (proliferative S-phase), C5 (proliferative M-S-phase), C6 (contractile SMCs), C7 (apoptotic SMCs), C8 (endothelial-like SMCs). B. Dot plot showing the expression of three genes defining each cell cluster shown in A. Color scale indicates log2 expression, and the size represents the percentage of cells. C. Integration of publicly available human coronary and carotid atherosclerotic plaques scRNA-seq datasets – with human aortic SMCs from this study. This analysis revealed 7 mesenchymal cell clusters (pC0 to pC6). The p stands for plaque. Analysis of human aortic SMCs in culture are shown in the background (light grey cluster). D. Human aortic SMCs (C0 to C8) are shown in front and human atherosclerotic cell populations are shown in the background (light grey clusters).
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    primary human aortic smcs  (ATCC)
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    ATCC primary human aortic smcs
    A. Outline of the experiment. Human <t>aortic</t> <t>SMCs</t> were exposed to static conditions (24 h), mechanical stretch (10%, 6h) or TNF stimulation (10 ng/mL, 24h). Uniform Manifold Approximation and Projection (UMAP) visualization of the phenotypic diversity of SMCs after static culture, stretch and TNF stimulation. Each dot represents a single cell. Nine cell clusters were identified in human aortic SMCs (0.3 resolution) as follows: C0 (fibromyocyte SMCs), C1 (inflammatory SMCs), C2 (proliferative M-phase), C3 (intermediate SMCs), C4 (proliferative S-phase), C5 (proliferative M-S-phase), C6 (contractile SMCs), C7 (apoptotic SMCs), C8 (endothelial-like SMCs). B. Dot plot showing the expression of three genes defining each cell cluster shown in A. Color scale indicates log2 expression, and the size represents the percentage of cells. C. Integration of publicly available human coronary and carotid atherosclerotic plaques scRNA-seq datasets – with human aortic SMCs from this study. This analysis revealed 7 mesenchymal cell clusters (pC0 to pC6). The p stands for plaque. Analysis of human aortic SMCs in culture are shown in the background (light grey cluster). D. Human aortic SMCs (C0 to C8) are shown in front and human atherosclerotic cell populations are shown in the background (light grey clusters).
    Primary Human Aortic Smcs, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+smcs/Primary+Aortic+Smooth+Muscle+Cells%3B+Normal%2C+Human/pm41067488-138-0-7
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    A. Outline of the experiment. Human aortic SMCs were exposed to static conditions (24 h), mechanical stretch (10%, 6h) or TNF stimulation (10 ng/mL, 24h). Uniform Manifold Approximation and Projection (UMAP) visualization of the phenotypic diversity of SMCs after static culture, stretch and TNF stimulation. Each dot represents a single cell. Nine cell clusters were identified in human aortic SMCs (0.3 resolution) as follows: C0 (fibromyocyte SMCs), C1 (inflammatory SMCs), C2 (proliferative M-phase), C3 (intermediate SMCs), C4 (proliferative S-phase), C5 (proliferative M-S-phase), C6 (contractile SMCs), C7 (apoptotic SMCs), C8 (endothelial-like SMCs). B. Dot plot showing the expression of three genes defining each cell cluster shown in A. Color scale indicates log2 expression, and the size represents the percentage of cells. C. Integration of publicly available human coronary and carotid atherosclerotic plaques scRNA-seq datasets – with human aortic SMCs from this study. This analysis revealed 7 mesenchymal cell clusters (pC0 to pC6). The p stands for plaque. Analysis of human aortic SMCs in culture are shown in the background (light grey cluster). D. Human aortic SMCs (C0 to C8) are shown in front and human atherosclerotic cell populations are shown in the background (light grey clusters).

    Journal: bioRxiv

    Article Title: Mechanical stretch regulates inflammatory signaling in human smooth muscle cells

    doi: 10.1101/2025.10.29.685276

    Figure Lengend Snippet: A. Outline of the experiment. Human aortic SMCs were exposed to static conditions (24 h), mechanical stretch (10%, 6h) or TNF stimulation (10 ng/mL, 24h). Uniform Manifold Approximation and Projection (UMAP) visualization of the phenotypic diversity of SMCs after static culture, stretch and TNF stimulation. Each dot represents a single cell. Nine cell clusters were identified in human aortic SMCs (0.3 resolution) as follows: C0 (fibromyocyte SMCs), C1 (inflammatory SMCs), C2 (proliferative M-phase), C3 (intermediate SMCs), C4 (proliferative S-phase), C5 (proliferative M-S-phase), C6 (contractile SMCs), C7 (apoptotic SMCs), C8 (endothelial-like SMCs). B. Dot plot showing the expression of three genes defining each cell cluster shown in A. Color scale indicates log2 expression, and the size represents the percentage of cells. C. Integration of publicly available human coronary and carotid atherosclerotic plaques scRNA-seq datasets – with human aortic SMCs from this study. This analysis revealed 7 mesenchymal cell clusters (pC0 to pC6). The p stands for plaque. Analysis of human aortic SMCs in culture are shown in the background (light grey cluster). D. Human aortic SMCs (C0 to C8) are shown in front and human atherosclerotic cell populations are shown in the background (light grey clusters).

    Article Snippet: Human aortic SMCs (ATCC, PCS-100-012, male donor) were authenticated by ATCC and tested negative for mycoplasma contamination prior to experiments.

    Techniques: Expressing